T2R2 Open-access full-text files surpass 4,000 mark!

The Tokyo Tech Research Repository, or T2R2, is a unified system for the registration, storage, management, and dissemination of papers, articles, books and other academic work published by researchers at Tokyo Tech, and the metadata (bibliographic information) for work published by individuals affiliated with the Institute. All materials in the system can be searched and read by users both on and off campus.

The number of T2R2 full-text files accessible to the public exceeded 4,000 on July 13, 2015. The T2R2 system will continue facilitating the dissemination of research being carried out at Tokyo Tech. We appreciate your continued use of the T2R2 system and your cooperation in data input and file registration.

The 4,000th paper registered in the T2R2 was published by Associate Professor Gen-ichi Konishi of the Department of Organic and Polymeric Materials, Graduate School of Science and Engineering.

Schematic view of stained mitochondria with pyrene dye
on cover of Journal of Materials Chemistry B, Vol.3, No.2

Title	Novel pyrene-based two-photon active fluorescent dye efficiently excited and emitting in the 'tissue optical window (650-1100 nm)'
Author	Yosuke Niko, Hiroki Moritomo, Hiroyuki Sugihara, Yasutaka Suzuki, Jun Kawamata and Gen-ichi Konishi
Journal	Journal of Materials Chemistry B
Volume, number, page	Vol. 3 No. 2 pp. 184-190

Comments from Associate Professor Konishi

Associate Professor
Gen-ichi Konishi

Two-photon fluorescence microscopy (TPFM) has received considerable attention as an advanced optical technique, especially in the biomedical field, because it can provide both in vivo and in vitro three-dimensional (3D) images deep within the samples of interest. The usability of this technique strongly depends on the performance of the probe molecules. We succeeded in synthesizing a new fluorescent dye, which exhibited outstanding properties such as bright fluorescence and a large two-photon (TP) absorption cross-section in the tissue optical window (650-1100 nm). In living mitochondria, pyrene (PY) provided more sensitive microscopic images than current dyes and showed great potential to be a building block of TP active fluorescent probes for the 1050 nm fiber laser.

In addition to researchers in chemical biology, medical science, and chemistry, I hope it will be read by researchers and students in other fields.

The team is currently focusing on the use of PY for not only in vitro cell imaging but also in vivo deep tissue imaging, using the 1050 nm fiber laser. In order to realize early detection for cancer and other pathological conditions, this technique can be applied to TPFM endoscopes.

• Konishi Laboratory
• New functional dye for realizing a versatile two-photon excitation microscope
• Tokyo Tech Research Repository (T2R2)
• T2R2 open access full-text files surpass 4,000 mark!
• T2R2 open-access full-text files surpass 2,500 mark!