<2004
Spring Semester>
Applied
Microbiology (2-0-0)
Even year
Prof.
Toshihisa ISHIKAWA
(Aim)
This
course addresses new technologies of Microbiology that can be applied for
medicine, drug discovery, protein manufacturing, and eco-business. Students are requested to present their
own report in English in the class.
The course involves lecture and debate/discussion regarding the
following items:
(Schedule)
I. Fundamentals
in microbiology
I-1.
Diversity of microorganisms
I-2
Classical application of microbiology
II. New
technologies of microbiology
II-1.
Concept of bio-factory
II-2.
Plasmids and vectors
II-3.
Synthesis of proteins
II-4.
Synthesis of vaccine
II-5.
Medical applications
III. Metabolites
of microorganisms
III-1.
Synthesis of antibiotics
III-2.
Synthesis of new drug seeds
III-3.
Transgenic microorganisms
IV. Application
of microbiology to eco-business
IV-1.
Microbiology and ecology
IV-2.
Eco-business and new challenges
Structure
and Function of Supramolecules (2-0-0) Even year
Associate
Prof. Fumio ARISAKA
(Aim)
Lectures will
focus on the assembly and function of supramolecules. After reviewing examples of supramolecules
in organisms, principles of the assembly will be described in details by taking
a virus or bacteriophages as an example. Also, the methodology of studying molecular
assembly, including electron microscopy and analytical ultracentrifugation
will be introduced.
(Schedule)
1.
Introduction to Supramolecules
Examples of
Supramolecules and the problems to be solved
2.
Hierarchy of Protein Structure
Primary to
Quaternery structure, Domain structure
3.
Repeating Units and Symmetry of Supramolecular Structure
Helical
Symmetry
4.
Domain Swapping
Origins of
Supramolecular Structure
5.
Structure of viruses
Classification
of viruses and bacteriophages
6.
Quasi-equivalence theory of Caspar & Klug
Icosahedral
symmetry and T (triangulation) number
7.
Molecular mechanism of adsorption and infection
Adsorption and
DNA injection
8.
Methods for studying virus assembly (1)
Use
of conditional lethal mutants and in vitro complementation
9.
Methods for studying virus assembly (2)
Expression
system of phage proteins
10.
Methods for studying virus assembly (3)
Biophysical
characterization of proteins and protein-protein interactions during assembly.
11.
Methods for studying virus assembly (4)
Structural
Genomics, Structural Biology and Biophysics of Virus Assembly
12.
Summary
Advanced
Cell Biochemistry
(2-0-0) Even year
Associate
Prof. Masayuki KOMADA
(Aim)
This course focuses on the mechanisms of protein trafficking and localization in eukaryotic cells, a traditional and attractive field in cell biology. Specific issues to be addressed are described below. Equal efforts will be made to address the fundamentals (first half) and recent findings (last half) in each lecture.
(Schedule)
1. Overview of protein trafficking
2. Endocytosis: how are cell surface receptors internalized into cells?
3. Protein sorting at the endosome: how are internalized receptors sorted for trafficking to the lysosome?
4. Protein sorting at the trans-Golgi network: how are newly-synthesized lysosomal hydrolases sorted for trafficking to the lysosome?
5. Rab family of small GTP-binding proteins: essential regulators of vesicular transport
6. Down-regulation of growth factor receptors: a mechanism to attenuate cell growth
signaling
7. Retroviral budding: how is the budding of retroviruses from infected cells related to
protein trafficking?
8. Autophagy: trafficking of organelles to the lysosome
9. Kinesin-mediated vesicular transport
10. Regulation of protein localization 1. phosphatidylinositol phosphates
11. Regulation of protein localization 2. PDZ domain
12. Regulation of protein localization 3. spectrin-actin membrane skeleton