Research

Tokyo Tech News

Protein cages for designing various catalytic reactions

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August 8, 2016

Compartmentalization is a common strategy used by living organisms to create isolated reaction environments to protect reaction catalysts from undesired reaction partners in cells. Mimicking such compartment systems is a novel approach for developing new biohybrid materials as well as for understanding the complex cellular processes.

Recently, the Ueno group at Tokyo Tech has developed a new strategy in which two different organometallic iridium (Ir) and palladium (Pd) metal complexes are immobilized into a single protein cage of apo-ferritin to construct a protein-based microcompartment.

The composite was prepared by the stepwise incorporation of Ir complex followed by introduction of Pd complex in buffer solution. The resulting protein composite was purified, crystallized, and characterized by X-ray crystallography.

The X-ray crystal structure analysis revealed that the Ir and Pd metal complexes have different binding preferences and to exist simultaneously inside the single protein cage. Notably, both the metal complexes are catalytically active and can promote cascade reactions such as Ir catalyzed hydrogenation and Pd catalyzed Suzuki-Miyaura cross-coupling reactions simultaneously inside the cage while keeping the entire cage structure intact.

The findings show that the concept of incorporating multiple metal catalysts into a single cage is promising for developing protein-based microcompartments and biomimetic materials for catalytic applications.

Schematic representation showing the crystal structure and catalytic reactions of apo-ferritin cage containing both Ir and Pd complexes.

Figure. Schematic representation showing the crystal structure and catalytic reactions of apo-ferritin cage containing both Ir and Pd complexes.

Reference

Authors:
B. Maity, K. Fukumori, S. Abe and T. Ueno
Title of original paper:
Immobilization of two organometallic complexes into a single cage to construct protein-based microcompartments
Journal:
Chem. Commun., 52, 5463-5466 (2016).
DOI :

School of Life Science and Technology

School of Life Science and Technology
—Unravel the Complex and Diverse Phenomena of Life—

Information on School of Life Science and Technology inaugurated in April 2016

School of Life Science and Technology

Schools, Departments, and Institute for Liberal Artsouter

Further information

Professor Takafumi Ueno
School of Life Science and Technology

Email tueno@bio.titech.ac.jp
Tel +81-45-924-5844

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This article has been updated to correct a typographical error on August 18.

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